The Payne Group
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Institute for Bioscience and Biotechnology Research (description) - About Gregory Payne
- Group Members' Areas of Research
- Biochip Collaborative Publications »
Featured Image: Electrode-imposed signals are used to assemble proteins without the need for reactive reagents. The two-step assembly approach uses 1) cathodic signals to electrodeposit the amino-polysaccharide chitosan and 2) anodic signals to activate the chitosan film for protein assembly. Proteins are shown to assemble at individual electrode addresses, with spatial selectivity and quantitative control.
The successful integration of biological components into microfabricated devices will provide "smarter" biosensors for diagnosing disease, detecting contaminants and discovering drugs. Integrating these biological components however will require hybrid fabrication methods that can exert spatial and temporal control during assembly while accommodating the labile nature of the bio-components (i.e., the nucleic acids, proteins, or cells).
The Payne Group is biofabricating by enlisting the unique properties of biological polymers e.g. chitosan) for two-way signal communication. Specifically, chitosan responds to localized electric signals from microfabricated devices and deposited as an interface for bio-components assembly. By reacting with selective substrate, the assembled biocomponents produce electric active substitutes and report the signal back to biomicrochips. This biosensor allows fast, high throughput and low limit detection of targeting biomeleculars.
Gregory Payne
Gregory Payne (Ph.D., University of Michigan, 1984) is a professor in the Fischell Department of Bioengineering, University of Maryland.
Dr.Payne is interested in building at nano-scales using enzymes and biopolymers. He has contributed with intelligent methodology to functionize surfaces of microfabricated devices with nanosized biocomponents.
Contact Information:
- Phone: (301) 405-8389
- E-mail: gpayne@umd.edu
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Group Members' Areas of Research
Dr. Yi Liu
Dr. Liu is developing novel fabrication methods to integrate biological components into micro-devices. He has demonstrated the signal transduction capability of stimuli-responsive biopolymers at the bio-device interface. Dr. Liu is also working on enzyme-mediated biomimetic approaches for macromolecular assembly.
Dr. Liu (Ph.D., Lehigh University, 2002) is a Faculty Research Associate at the Institute for Bioscience and Biotechnology Research. He also holds a B.S. in Chemistry from Peking University, Beijing, China.
Dr. Mikhael Wallowitz
Dr. Wallowitz is working on assembling immunoassays onto pattered electrodes using biological polymers. Currently he is developing novel approaches for improving immunoassays by reducing incubation time and enhancing detection using electrochemical techniques.
Dr. Wallowitz (Ph.D., UC Davis, 2006) is a Faculty Research Associate at the Institute for Bioscience and Biotechnology Research. He also holds a B.S. from the University of Connecticut and M.S. from Texas A&M University.
Dr. Eunkyoung Kim
Dr. Kim has been interested in integrating the electrochemistry into the biology. Recently, she is studying in the electrochemical properties of phenol-grafted chitosan because catechol-quinone redox couples are commonly used in biology to mediate electron transfer. A catechol-grafted chitosan mimicked the electron transfer mediated in biology. The biomimetic film is fabricated by grafting catechol onto the aminopolysaccharide chitosan using an electrochemical method to impart electrochemical properties. Specifically, the catechol-modified chitosan films can perform amplification, partial rectification and switching functions. In the future, she will study the potential biological precedents for electrochemically-active films.
Dr. Kim (Ph.D., Electrochemistry in Korea Advanced Institute of Science and Technology, 2003) is a Faculty Research Associate at the Institute for Bioscience and Biotechnology Research.
Past Researchers
Dr. Xiaowen Shi
Dr. Shi worked on biomolecular assembly onto integrated biochips. He developed new methods to connect proteins to electrodeposited chitosan (e.g., tyrosinase conjugation, metal affinity binding, biotin-avidin conjugation). He also used localized electric signals to guide protein assembly onto activated chitosan film, as shown in the scheme below, allowing spatially and quantitatively control of protein assembly onto patterned biochips. In addition, he developed methods to assemble cells onto biochips using localized electric signals.
Dr. Shi received his Ph.D. in 2006 from Wuhan University. He also holds a B.S. and M.S. from Wuhan University.
Xiaohua Yang
Xiaohua worked with enzyme-mediated assembly of biomolecular components onto microfabricated electronic devices and signal recognition based on biomolecules interaction. He is developed methods to: 1) integrate biomolecules onto electrodeposited chitosan film catalyzed by enzymes such as tyrosinase and microbial transglutaminase; 2) capture and recognize signals from biomolecular interaction and 3) transform the recognition into electric or optical signal.