The Payne Group
- Based in:
The Center for Biosystems Research (description | web site ») - About Gregory Payne
- Group Members' Areas of Research
- Biochip Collaborative Publications »
Featured Image: Electrode-imposed signals are used to assemble proteins without the need for reactive reagents. The two-step assembly approach uses 1) cathodic signals to electrodeposit the amino-polysaccharide chitosan and 2) anodic signals to activate the chitosan film for protein assembly. Proteins are shown to assemble at individual electrode addresses, with spatial selectivity and quantitative control.
The successful integration of biological components into microfabricated devices will provide "smarter" biosensors for diagnosing disease, detecting contaminants and discovering drugs. Integrating these biological components however will require hybrid fabrication methods that can exert spatial and temporal control during assembly while accommodating the labile nature of the bio-components (i.e., the nucleic acids, proteins, or cells).
The Payne Group is biofabricating by enlisting the unique properties of biological polymers e.g. chitosan) for two-way signal communication. Specifically, chitosan responds to localized electric signals from microfabricated devices and deposited as an interface for bio-components assembly. By reacting with selective substrate, the assembled biocomponents produce electric active substitutes and report the signal back to biomicrochips. This biosensor allows fast, high throughput and low limit detection of targeting biomeleculars.
Gregory Payne
Gregory Payne (Ph.D., University of Michigan, 1984) is a professor at the University of Maryland Biotechnology Institute (UMBI) and the director of its Center for Biosystems Research (CBR).
Dr.Payne is interested in building at nano-scales using enzymes and biopolymers. He has contributed with intelligent methodology to functionize surfaces of microfabricated devices with nanosized biocomponents.
Contact Information:
- Phone: (301) 405-8389
- E-mail: payne@umbi@umd.edu
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Group Members' Areas of Research
Dr. Yi Liu
Dr. Liu is studying signal transduction using chitosan-coated electrode. He has developed a novel approach that enables electrochemically-initiated reactions to generate optical signals that can be used to enhance the discriminating power for the analysis of antioxidant food phenols. Yi is currently exploring the transduction properties of chitosan-coated wires functionalized with enzymes. He is also working on biomimetic approaches for macromolecular assembly.
Dr. Liu (Ph.D., Lehigh University, 2002) is a Faculty Research Associate at the Center for Biosystems Research, University of Maryland Biotechnology Institute. He also holds a B.S. in Chemistry from Peking University, Beijing, China.
Dr. Xiaowen Shi
Dr. Shi is working on biomolecular assembly onto integrated biochips. He is developing new methods to connect proteins to electrodeposited chitosan (e.g., tyrosinase conjugation, metal affinity binding, biotin-avidin conjugation). Currently, he is using localized electric signals to guide protein assembly onto activated chitosan film, as shown in the scheme. This allows spatially and quantitatively control of protein assembly onto patterned biochips. In addition, he is developing methods to assemble cells onto biochips using localized electric signals.
Dr. Shi (Ph.D. 2006, Wuhan University) is a Faculty Research Associate at the Center for Biosystems Research, University of Maryland Biotechnology Institute. He also holds a B.S. and M.S. from Wuhan University.
Xiaohua Yang
Xiaohua is interested in enzyme-mediated assembly of biomolecular components onto microfabricated electronic devices and signal recognition based on biomolecules interaction. Currently, he is developing methods to: 1) integrate biomolecules onto electrodeposited chitosan film catalyzed by enzymes such as tyrosinase and microbial transglutaminase; 2) capture and recognize signals from biomolecular interaction and 3) transform the recognition into electric or optical signal.